Ethylenediaminetetraacetic acid ( EDTA ) anticoagulant affects the refractometric measurement of total protein concentration in equine synovial fluid

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EDTA (Ethylenediaminetetraacetic Acid)

Ethylenediaminetetraacetic acid (EDTA) was developed by Franz Munz inGermanyduring the 1930s as an alternative to citric acid. About the same time, Frederick Bersworth synthesized EDTA in the United States using a different technique. He obtained aUSpatent for this technique in1945. EDTAwas approvedby the US Food and Drug Administration (FDA) as a food additive in 1947. Since the early 1950s, E...

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Concentration of hyaluronic acid in synovial fluid.

ISOHE (1) HAS DESCRIBED a color reaction produced by the addition of alcoholic carbazole to uronic acids previously heated in 87% sulfuric acid. The purpose of the present report is to apply a modification of this method to the determination of hyaluronic acid in synovial fluid. The concentration of mucin in synovial fluid has been determined by the turbidity of the mucin clot (2) or by the che...

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Cerebrospinal fluid nerve growth factor and total protein concentration in the children with meningitis

Meningitis is one of the most common infectious cerebral nervous system (CNS), defined as an inflammation of the meninges. It is clinically categorized into a chronic and acute based on the acuity of symptoms. Vomiting, bulging fontanel and fever are the main symptoms in the patients with meningitis. Bacterial meningitis is a severe, potentially life-threatening infection that is associated wit...

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48, XXYY syndrome associated with ethylenediaminetetraacetic acid (EDTA)-dependent pseudothrombocytopenia.

A 56-yr-old man with hypogonadism, gynecomastia, and mental retardation was evaluated for chromosome constitution and thrombocytopenia. Chromosomal analysis demonstrated the mosaicism of 48, XXYY and 47, XXY in the peripheral lymphocytes. Twenty out of twenty-five cells were 48, XXYY karyotype and the remaining five were 47, XXY karyotype. Thrombocytopenia was the EDTA-dependent pseudothrombocy...

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Synovial fluid total protein concentration as a possible marker for canine idiopathic polyarthritis

Idiopathic polyarthritis (IPA) is a very common inflammatory arthropathy in the dog. Canine IPA is diagnosed mainly by detecting increased number of leukocytes in the synovial fluid (SF), which is easily influenced by glucocorticoid therapy. We obtained 31 SF samples from 24 IPA dogs prior to (n=19) and/or after (n=12) 1 to 10 weeks of glucocorticoid therapy. The SF total protein concentrations...

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ژورنال

عنوان ژورنال: Equine Veterinary Education

سال: 2020

ISSN: 0957-7734,2042-3292

DOI: 10.1111/eve.12971